| Histology Protocols 2010 Edition Contributor(s): Hewitson, Tim D. (Editor), Darby, Ian A. (Editor) |
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ISBN: 1603273441 ISBN-13: 9781603273442 Publisher: Humana OUR PRICE: $104.49 Product Type: Hardcover - Other Formats Published: December 2009 |
| Additional Information |
| BISAC Categories: - Medical | Histology - Science | Life Sciences - Molecular Biology - Medical | Laboratory Medicine |
| Dewey: 611 |
| Series: Methods in Molecular Biology |
| Physical Information: 0.56" H x 7" W x 10" (1.40 lbs) 229 pages |
| Descriptions, Reviews, Etc. |
| Publisher Description: Somuchofwhatweknowaboutthepathogenesisofhumandiseasehascomefromthe systematic and careful study of histological material. Indeed, every internal medicine discipline has its landmark papers describing the clinico-pathological correlations. However, increasingly, it is molecular and cellular biology that provides the necessary mechanistic insights. For many years, it was thought that the two skill sets were mutually exclusive, but we hope that this book shows that this is not necessarily so. Implicitinthescienceofhistologyisthepreservationandarchivingoftissue.PartIof the book concentrates on the preparation of tissue, providing an overview of fixation, embedding, and processing (Chapter 1), and in Chapters 2 and 3, the required techniques for the retrieval of RNA from histological sections. Both routine and specialist histological staining techniques are provided in Part II. These include pro- cols for immuno (Chapters 4-7), lectin (Chapter 8), and hybridization (Chapter 9) histochemistry, histologicalstaining (Chapters10and11), aswellasspecificmethods for the in situ identification of hypoxia (Chapter 12) and apoptosis (Chapter 13). Finally, Part III details advances in imaging (Chapters 14-16) and image analysis (Chapter 17). It is hoped that this volume will provide molecular biologists with the basic his- chemical techniques and histologists with the molecular techniques to realise the potential of their resource. We are indebted to the authors for their generosity in sharing these protocols. |