| 2D Page: Sample Preparation and Fractionation: Volume 1 Contributor(s): Posch, Anton (Author) |
|
 |
ISBN: 1617377422 ISBN-13: 9781617377426 Publisher: Humana OUR PRICE: $151.99 Product Type: Paperback - Other Formats Published: December 2010 |
| Additional Information |
| BISAC Categories: - Science | Life Sciences - Biochemistry - Science | Life Sciences - Cell Biology - Science | Life Sciences - Microbiology |
| Dewey: 572.6 |
| Series: Methods in Molecular Biology |
| Physical Information: 0.96" H x 6.14" W x 9.21" (1.46 lbs) 459 pages |
| Descriptions, Reviews, Etc. |
| Publisher Description: This book, split into two volumes, presents a broad coverage of the principles and recent developments of sample preparation and fractionation tools in Expression Proteomics in general and for two-dimensional electrophoresis (2-DE) in particular. 2-DE, with its unique capacity to resolve thousands of proteins in a single run, is still a fundamental research tool for nearly all protein-related scientific projects. The methods described here in detail are not limited to 2-DE and can also be applied to other protein separation techniques. Because each biological sample is unique, a suited sample preparation strategy has to consider the type of sample as well as the type of biological question being addressed. The complex nature of proteins often requires a multitude of sample preparation options. In addition, sample preparation is not only a prerequisite for a successful and reproducible Proteomics exp- iment, but also the key factor to meaningful data evaluation. Interestingly, not much attention was paid to this area during Proteomics methodology dev- opment and therefore this book is intended to explain in depth how proteins from various sources can be properly isolated and prepared for reproducible Proteome analysis. |