Advanced Photon Counting: Applications, Methods, Instrumentation Softcover Repri Edition Contributor(s): Kapusta, Peter (Editor), Wahl, Michael (Editor), Erdmann, Rainer (Editor) |
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ISBN: 3319354299 ISBN-13: 9783319354293 Publisher: Springer OUR PRICE: $427.49 Product Type: Paperback - Other Formats Published: October 2016 |
Additional Information |
BISAC Categories: - Science | Spectroscopy & Spectrum Analysis - Technology & Engineering | Lasers & Photonics - Science | Physics - Atomic & Molecular |
Dewey: 571.4 |
Series: Springer Series on Fluorescence (Hardcover) |
Physical Information: 0.79" H x 6.14" W x 9.21" (1.18 lbs) 370 pages |
Descriptions, Reviews, Etc. |
Publisher Description: This volume focuses on Time-Correlated Single Photon Counting (TCSPC), a powerful tool allowing luminescence lifetime measurements to be made with high temporal resolution, even on single molecules. Combining spectrum and lifetime provides a "fingerprint" for identifying such molecules in the presence of a background. Used together with confocal detection, this permits single-molecule spectroscopy and microscopy in addition to ensemble measurements, opening up an enormous range of hot life science applications such as fluorescence lifetime imaging (FLIM) and measurement of F rster Resonant Energy Transfer (FRET) for the investigation of protein folding and interaction. Several technology-related chapters present both the basics and current state-of-the-art, in particular of TCSPC electronics, photon detectors and lasers. The remaining chapters cover a broad range of applications and methodologies for experiments and data analysis, including the life sciences, defect centers in diamonds, super-resolution microscopy, and optical tomography. The chapters detailing new options arising from the combination of classic TCSPC and fluorescence lifetime with methods based on intensity fluctuation represent a particularly unique highlight. |