| Genetic Library Construction and Screening: Advanced Techniques and Applications 2002 Edition Contributor(s): Bird, R. C. (Editor), Smith, Bruce F. (Editor) |
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ISBN: 3540672788 ISBN-13: 9783540672784 Publisher: Springer OUR PRICE: $94.05 Product Type: Spiral - Other Formats Published: March 2002 |
| Additional Information |
| BISAC Categories: - Medical | Laboratory Medicine - Science | Life Sciences - Molecular Biology - Science | Life Sciences - Biochemistry |
| Dewey: 571.6 |
| LCCN: 2002728113 |
| Series: Springer Lab Manuals |
| Physical Information: 241 pages |
| Descriptions, Reviews, Etc. |
| Publisher Description: Designed as an introductory text the authors cover all core strategies in the application of modern recombinant DNA technology. The first chapters directly address the applications of polymerase chain reaction to a variety of problems in DNA cloning that are, or have been, extremely challenging using more traditional approaches and technologies. These include cDNA cloning and transcript mapping, mutagenesis as well as the cloning of very long transcripts and protocols using limiting amounts of total RNA. Further chapters describe approaches to subtractive cloning technologies as well as novel specialized expression cloning and library screening strategies. The handbook contains detailed step-by-step protocols and extensive hands-on advice. |