| Alterations of the Sister Chromatid Exchange frequency in peripheral lymphocytes caused by an Ironman triathlon Contributor(s): Meisel, Marlies (Author) |
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ISBN: 3638910415 ISBN-13: 9783638910415 Publisher: Grin Verlag OUR PRICE: $59.76 Product Type: Paperback Published: February 2008 |
| Additional Information |
| BISAC Categories: - Law | Wills - Medical | Clinical Medicine - Medical | Genetics |
| Physical Information: 0.22" H x 5.83" W x 8.27" (0.29 lbs) 92 pages |
| Descriptions, Reviews, Etc. |
| Publisher Description: Diploma Thesis from the year 2007 in the subject Medicine - Human Genetics, grade: 1,0, University of Vienna (Institut f r Ern hrungswissenschaften), 96 entries in the bibliography, language: English, abstract: The aim of the present study was to investigate the effect of a triathlon (3.8 km swim, 180 km cycle, 42,2 km run) on the genomic stability of nine highly trained non- professional athletes. Therefore, the SCE assay, a relevant biological response marker for genotoxicity in human biomonitoring studies PENDZICH et al., 1997] was performed using peripheral lymphocytes, on account of their effortless accessibility WILKOSCY and RYNARD, 1990]. Duplicate lymphocyte cell cultures, of each participant, were incubated for 72 h (37 C, 5% CO2) according to a short-term human lymphocyte cell culture. For each participant at least 50 metaphases, containing 43-46 chromosomes were scored, to evaluate the mean SCE frequency. The number of SCEs per cell was calculated to a chromosome set of a normal diploid human cell, containing 46 chromosomes. In the present study the alteration of SCE frequency, 48 h pre- and 24 h postrace was evaluated. As an additional endpoint Top 5 HFCs (highest five absolute SCE means) were assayed. It could be demonstrated that both the total mean SCE frequency and the mean Top 5 HFC frequency (n=9) 24 h postrace were significantly decreased (*p |